Table 3.
Antibodies condition used in the Western blot. Milk: M, overnight: O/N, room temperature: RT, bovine serum albumin: BSA.
| Primary Antibody | Secondary Antibody | |
|---|---|---|
| mBDNF (14kDa) | 1:1000 M 3% (Icosagen), 4° O/N | Anti-mouse1:3000 M3%, 1h RT |
| TRKB (145–95 kDa) | 1:750 M5% (Cell signaling), 4° O/N | Anti-rabbit 1:2000 M5%, 1h RT |
| TRKB Tyr 706 (145 kDa) | 1:500 M5% (Cell signaling), 4° O/N | Anti-rabbit 1:1000 M5%, 1h RT |
| AKT Ser473 (60 kDa) | 1:1000 M5% (Cell signaling), 2h RT | Anti-rabbit 1:1000 M5%, 1h RT |
| AKT (60 kDa) | 1:1000 M10% (Cell signaling), 4° O/N | Anti-rabbit 1:1000 M5%, 1h RT |
| pS6 Ser240/244 (32 kDa) | 1:1000 M5% (Cell signaling), 4° O/N | Anti-rabbit 1:2000 M5%, 1h RT |
| S6 (32 kDa) | 1:1000 M5% (Cell signaling), 4° O/N | Anti-rabbit 1:2000 M5%, 1h RT |
| pERK1/2 Thr202/ Tyr204 (44 kDa) | 1:1000 M3% (Cell signaling), 4° O/N | Anti-rabbit 1:2000 M3%, 1h RT |
| ERK1/2 (44 kDa) | 1:1000 M3% (Santa Cruz Biotechnology), 4° O/N | Anti-rabbit 1:5000 M3%, 1h RT |
| pPLCγ Tyr783 (155 kDa) | 1:1000 BSA5% (Cell signaling), 4° O/N | Anti-rabbit 1:2000 M5%, 1h RT |
| PLCγ (155 kDa) | 1:1000 M5% (Cell signaling), 4° O/N | Anti-rabbit 1:1000 M5%, 1h RT |
| β-ACTIN (43 kDa) | 1:10,000 M3% (Sigma-aldrich), 4° O/N | Anti-mouse 1:10,000 M3%, 1h RT |