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. 2020 Aug 28;21(17):6244. doi: 10.3390/ijms21176244

Figure 4.

Figure 4

THC treatment decreases T cell activation and alters metabolism to induce apoptosis. Spleen cells were cultured with SEB+THC or SEB+Veh for 72 h, followed by the purification of CD3+ T cells, as described in Methods. (A): OCR in cells during the mitochondrial stress test. (B): OCR in the glucose oxidation dependency test. (C): OCR in the β-oxidation dependency test. (D): Heat map showing dysregulated apoptosis-related metabolites from metabolome analysis of serum (n = 4). (E–H): Concentrations of metabolites in serum from SEB+Veh and SEB+THC mice. (I): DiOC6(3) staining of splenocytes from C3H/HeJ mice activated with SEB (1 μg/mL) in the presence of either vehicle or PLC 200 μM for 72 h. (J): T cell proliferation measured by 3-H thymidine incorporation assay in splenocytes from C3H/HeJ mice activated with SEB (1 µg/mL) in the presence of either vehicle or PLC 200 µm for 72 h. (CPM, counts per minute). Statistical significances are depicted as * p < 0.05, ** p < 0.01, and **** p < 0.0001 between the groups.