Table 1.
Host range of phages vB_SenM-1, vB_SenM-2, and vB_SenS-3.
Bacterial Strain | Sensitivity to Phage c | |||
---|---|---|---|---|
Salmonella enterica Serotype a | Source and Identification Number b | vB_SenM-1 | vB_SenM-2 | vB_SenS-3 |
Heidelberg | DMB UG collection; no. UGSA2 | 100% | 100% | 100% |
Panama | DMB UG collection; no. UGSA3 | 97.82% ± 3.28% | 93.23% ± 4.21% | 95.12% ± 6.16% |
Reading | DMB UG collection; no. UGSA4 | <0.01% | <0.01% | 15.21% ± 1.11% |
London | DMB UG collection; no. UGSA5 | <0.01% | <0.01% | 1.25% ± 0.09% |
Anatum | NSC, no. 78 | 28.11% ± 3.16% | 95.12% ± 4.11% | <0.01% |
Typhimurium * | NSC, no. 12 | 67.13% ± 7.28% | 98.20% ± 4.77% | 95.13% ± 2.22% |
Typhimurium | NSC, no. 13 | 23.28% ± 1.70% | 22.40% ± 3.41% | 98.61% ± 1.17% |
Stanley * | NSC, no. 15 | 0.80% ± 0.06% | 97.28% ± 2.16% | 0.63% ± 0.11% |
Heidelberg | NSC, no. 16 | 27.91% ± 8.09% | 98.66% ± 1.06% | 100% ± 1.03% |
Cholerasuis | NSC, no. 34 | 17.22% ± 0.47% | 97.81% ± 3.22% | 99.82% ± 7.57% |
Cholerasuis | NSC, no. 39 | 4.80% ± 0.88% | 89.16% ± 7.22% | 93.27% ± 4.21% |
Cholerasuis var kunznedorf | NSC, no. 37 | 88.46% ± 2.61% | 80.52% ± 7.36% | 93.17% ± 4.16% |
Cholerasuis | NSC, no. 1439 | <0.01% | <0.01% | 9.11% ± 0.76% |
Virchow | NSC, no. 41 | <0.01% | <0.01% | <0.01% |
Newport | NSC, no. 50 | 17.26% ± 2.16% | 10.08% ± 3.25% | <0.01% |
Newport | NSC, no. 51 | <0.01% | <0.01% | 98.26% ± 3.89% |
Enteritidis * | NSC, no. 64 | 88.87% ± 6.12% | 95.18% ± 2.16% | 98.26% ± 3.15% |
Enteritidis * | NSC, no. 1392 | 66.21% ± 4.15% | 81.22% ± 4.33% | 86.36% ± 2.53% |
Dublin | NSC, no. 65 | 55.21% ± 1.17% | 88.25% ± 3.21% | 97.16% ± 5.21% |
Gallinarum | NSC, no. 74 | 63.55% ± 2.11% | 91.66% ± 5.11% | 89.21% ± 6.10% |
Seftenberg | NSC, no. 87 | <0.01% | <0.01% | 94.66% ± 2.22% |
Infantis * | NSC, no. 155 | 10.71% ± 1.06% | <0.01% | 6.15% ± 0.91% |
Bovismorbificans * | NSC, no. 300 | <0.01% | <0.01% | 94.27% ± 2.31% |
Saintpaul | NSC, no. 435 | 63.28% ± 4.95% | 96.21% ± 5.13% | 89.92% ± 2.89% |
Kentucky * | NSC, no. 1368 | 51.22% ± 7.33% | 49.97% ± 3.47% | 24.25% ± 2.97% |
Agona * | NSC, no. 1408 | 29.18% ± 3.15% | 98.36% ± 3.67% | 95.22% ± 5.11% |
Hadar * | NSC, no. 1784 | <0.01% | <0.01% | 7.66% |
a Resistance to the following antibiotics was tested using minimal inhibitory concentration (MIC) and disc diffusion tests: tetracycline, rifampicin, ampicillin, chloramphenicol, colistin, enrofloxacin, and kanamycin. The resistance/susceptibility was determined on the basis of EUCAST guidelines. Investigated strains were susceptible to all tested antibiotics, with the exception of colistin for strains indicated by asterisks. b Abbreviations: NSC, National Salmonella Centre, Poland; DMB UG, Department of Molecular Biology of University of Gdansk, Poland. c Phage titer on S. Heidelberg UGSA2 was used as a reference value (100%). Mean values from three independent experiments are shown with SD.