Table 2.
Primer sequences for real-time polymerase chain reaction (RT-qPCR) analysis.
| Target (Gene Name) | Primer | Sequence 5′→3′ | Size, bp |
|---|---|---|---|
| SIRT1 | Std-curve-F | agctgatgaaccgcttgctat | 300 |
| Std-curve-R | ttggcatattcaccacctaacc | ||
| qPCR-F | ttgttattgggtcttccctcaaa | 112 | |
| qPCR-R | aaatgcagatgaggcaaaggtt | ||
| CLDN1 | Std-curve-F | agcacattgcaagcaacccgtgcct | 320 |
| Std-curve-R | agggcacctcccagaaggcagaga | ||
| qPCR-F | ccgttggcatgaagtgtatg | 101 | |
| qPCR-R | agccagacctgcaagaagaa | ||
| ACTB | Std-curve-F | gaggcatcctcaccctgaagta | 232 |
| Std-curve-R | acagcctggatagcaacgtaca | ||
| qPCR-F | tggagaaaatctggcaccac | 125 | |
| qPCR-R | catgatctgggtcatcttctcg | ||
| B2M | Std-curve-F | ctactctctctttctggcctggag | 511 |
| Std-curve-R | aaacatggagacagcactcaaagt | ||
| qPCR-F | tccagcgtactccaaagattc | 113 | |
| qPCR-R | gtcaacttcaatgtcggatgg |
SIRT1: Sirtuin 1 gene; CLDN1: Claudin 1 gene; ACTB: actin β gene; B2M: β-2-microglobulin gene; Std-curve-F/R: Standard curve forward or reverse primer; qPCR-F/R: quantitative real time PCR forward or reverse primer.