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. 2020 Aug 23;21(17):6066. doi: 10.3390/ijms21176066

Figure 3.

Figure 3

In vitro maturation of ARPE-19 upregulates the expression of therapeutically relevant photoreceptor and choroid trophic factors. (a) RT-qPCR analysis of mRNA levels of key RPE genes in mature ARPE-19 cells (>42 days in culture; n = 4). The results were normalized to an endogenous reference gene (PPIA) and are presented as mean fold change (2−ΔΔCT) relative to immature ARPE-19 cultures (dotted line) ± standard deviation. Data were compared with a Mann–Whitney U test of ΔCt values; * p < 0.05; NS: not significant. (b) Protein secreted by the post-confluent (PC) immature and mature ARPE-19 cultures into the conditioned media presented as mean concentration ± standard deviation (n = 5), Mann–Whitney U test.