. 2020 Aug 23;21(17):6066. doi: 10.3390/ijms21176066

Table A1.

RT-qPCR primers chosen to evaluate the functions of human RPE.

Gene	Primers	Function	Reference
PEDF	TCATTCACCGGGCTCTCTACT	Antiangiogenesis and antiapoptotic photoreceptor trophic factor with a potent neurotrophic effect.	[72]
PEDF	GGGCAGTGACCGTGTCAAG		[72]
IGF1	GCTCTTCAGTTCGTGTGTGGA	Anti-apoptotic and photoreceptor prosurvival factor.	[36]
IGF1	GCCTCCTTAGATCACAGCTCC	Anti-apoptotic and photoreceptor prosurvival factor.	[36]
FGF2	AGAAGAGCGACCCTCACATCA	Neurotrophic and angiogenic factor required for photoreceptor and choroid maintenance.	[73]
FGF2	CGGTTAGCACACACTCCTTTG		[73]
BDNF	CTACGAGACCAAGTGCAATCC	Neurotrophic factor with a prosurvival effect on photoreceptors.	[50]
BDNF	AATCGCCAGCCAATTCTCTTT		[50]
GAS6	CCGGGGACTTGTTCCAACC	Involved in the phagocytosis of photoreceptor outer segments.	[74]
GAS6	CTGCACGAGGTCCTTCTCAT		[74]
FASL	TGCCTTGGTAGGATTGGGC	Antiangiogenic factor that also allows RPE to uphold and maintain the immune privilege feature of the eye by inactivating immune cells.	[40,75]
FASL	GCTGGTAGACTCTCGGAGTTC		[40,75]
TGFB	CAATTCCTGGCGATACCTCAG	Angiogenic factor that induces VEGF secretion by RPE and choroid cells.	[41]
TGFB	GCACAACTCCGGTGACATCAA		[41]
TIMP3	TGGGTTGTAACTGCAAGATCAAG	Involved in the maintenance and remodeling of Bruch’s membrane.	[76]
TIMP3	GGTCCAGAGACACTCGTTCT		[76]
VEGFA	AGGGCAGAATCATCACGAAGT	Angiogenic factor secreted by RPE to maintain the choriocapillaris.	[9]
VEGFA	AGGGTCTCGATTGGATGGCA		[9]
PDGFA	GCAAGACCAGGACGGTCATTT	Angiogenic factor with an autocrine growth effect on RPE.	[77,78]
PDGFA	GGCACTTGACACTGCTCGT	Angiogenic factor with an autocrine growth effect on RPE.	[77,78]
KDR	GTGATCGGAAATGACACTGGAG	VEGF receptor and plays a crucial role eye development.	[79]
KDR	GTGATCGGAAATGACACTGGAG	VEGF receptor and plays a crucial role eye development.	[79]
BEST1	CTGGGCTTCTACGTGACGC	Calcium-activated anion channel and a regulator of intracellular calcium signaling.	[44,80]
BEST1	TTGCTCGTCCTTGCCTTCG		[44,80]
CCL2	CAGCCAGATGCAATCAATGCC	Chemokine involved in the regulation and modulation of an immune response.	[81]
CCL2	TGGAATCCTGAACCCACTTCT		[81]
CFH	GTGAAGTGTTTACCAGTGACAGC	Regulates and modulates the complement pathway.	[82]
CFH	AACCGTACTGCTTGTCCAAAA	Regulates and modulates the complement pathway.	[82]
FGF2R	AGCACCATACTGGACCAACAC	FGF-2 receptor.	[46]
FGF2R	GGCAGCGAAACTTGACAGTG	FGF-2 receptor.	[46]
CXCL8	ACTGAGAGTGATTGAGAGTGGAC	Encodes a chemokine (IL-8) that initiates and amplifies inflammation. It has also been implicated in the pathogenesis of AMD.	[65]
CXCL8	AACCCTCTGCACCCAGTTTTC		[65]
RPE65	CCTGCTGGTGGTTACAAGAAA	Encodes an essential isomerohydrolase enzyme in the retinoid visual cycle.	[43]
RPE65	CCTGCCTGTTACATGAGCTGT		[43]
LHX2	ATGCTGTTCCACAGTCTGTCG	Highly involved in early eye development and regulates the expression of multiple visual cycle genes.	[83,84]
LHX2	GCATGGTCGTCTCGGTGTC		[83,84]
LOXL1	CCACTACGACCTACTGGATGC	RPE signature gene that has been reported to be involved in ocular disorders.	[85]
LOXL1	GTTGCCGAAGTCACAGGTG		[85]
MYRIP	CTAAGAGCGGGACGTTTCAGG	Encodes for a protein that enables the trafficking of melanosomes in RPE.	[86]
MYRIP	TCTTCCTCGCTATCGGAGCC		[86]
TRPM1	GTTCACCAACCAGCATATCCC	Encodes a permeable cation channel and plays a role in melanin synthesis in the RPE.	[46]
TRPM1	AGCCTTGATCAGGCCTTTCC		[46]
CNTF	ACAGAGCATTCACCGCTGAC	Encodes neurotrophic cytokine with a photoreceptor prosurvival effect. It was determined to be the most stable gene between the 2D and 3D RPE cultures.	[38]
CNTF	TCAGGTCTGAACGAATCTTCCTT		[38]