Table 2.
Percentage of decrease (↓) or increase (↑) of sialylated glycan determinants in Capan-1 KD cells vs. the corresponding control (scramble) cells determined by flow cytometry.
| Cells | % change in sLex | Sig.1 | Sig.1 | Sig.1 | % change in sLea | Sig.1 | Sig.1 | Sig.1 | % change in α2,6-SA | Sig.1 | Sig.1 | Sig.1 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| vs. SC cells 1 | vs. shST3GAL4_1 | vs. shST3GAL4_4 | vs. shST3GAL3_7 | vs. SC cells 1 | vs. shST3GAL4_1 | vs. shST3GAL4_4 | vs. shST3GAL3_7 | vs. SC cells 1 | vs. shST3GAL4_1 | vs. shST3GAL4_4 | vs. shST3GAL3_7 | |
| shST3GAL4_1 Capan-1 | ↓64% (***) | - | ↓32% (*) | - | ↑35% (**) | - | ||||||
| shST3GAL4_4 Capan-1 | ↓63% (***) | ns | - | ↓45 (**) | ns | - | ↑24% (*) | ns | - | |||
| shST3GAL3_7 Capan-1 | ↓61% (***) | ns | ns | - | ↓43% (**) | ns | ns | - | ↑20% (ns) | ns | ns | - |
| shST3GAL3_9 Capan-1 | ↓73% (***) | ns | ns | ns | ↓52% (***) | ns | ns | ns | ↑12% (ns) | * | ns | ns |
1 ANOVA and Tukey’s multiple comparison post-hoc test was performed. ns: not significant; p < 0.05: *; p < 0.01: ** and p < 0.001:***.