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. 2020 Aug 20;21(17):6007. doi: 10.3390/ijms21176007

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Labeling efficiency and cytotoxicity of 6-Az-Fuc. (A) Neuro2A cells were treated with DMSO, 100 μM peracetylated 6-Alk-Fuc, 7-Alk-Fuc or 6-Az-Fuc. Incorporated Fuc analogs in the cell lysates were biotinylated by click chemistry, and the labeled glycans on proteins were detected by blotting with HRP-streptavidin. For labeling, the same concentrations of biotin-azide (lane 1–3) and biotin-alkyne (lane 4 and 5) were used. (B) Growth of HEK293 and Neuro2A cells was examined after addition of DMSO or peracetylated 6-Az-Fuc at Day 0 (n = 3). All graphs show means ± SD.