Table 5.

Sensitivity and accuracy of anthrone quantification method for cellulose-derived glucose.

	Theoretical ng µL−1 Glucose	Measured ng µL−1 Glucose	Standard Deviation	RME (%)	LOD ng µL−1	LOQ ng µL−1	Equation R2
Day 1 n = 4	6.67	7.15	0.036	7.23	2.62	8.72	y = 0.032x + 0.0260 R2 = 0.9969
	13.33	13.11	0.055	−1.70
	20.00	19.73	0.060	−1.35
	26.67	25.96	0.051	−2.67
	33.33	34.06	0.072	2.17
Day 2 n = 4	6.67	7.41	0.032	11.09	1.03	3.45	y = 0.0312x + 0.0326 R2 = 0.9966
	13.33	12.58	0.029	−5.67
	20.00	19.49	0.025	−2.55
	26.67	27.00	0.065	1.23
	33.33	33.53	0.078	0.59
Day 3 n = 4	6.67	7.47	0.014	12.11	3.00	9.99	y = 0.0311x + 0.0339 R2 = 0.9920
	13.33	13.22	0.043	−0.83
	20.00	18.43	0.014	−7.87
	26.67	26.92	0.015	0.94
	33.33	33.96	0.072	1.88
Average n = 12	6.67	7.34	0.014	10.12	2.22	7.40	y = 0.0314x + 0.0225 R2 = 0.9967
	13.33	12.97	0.043	−2.72
	20.00	19.22	0.014	−3.90
	26.67	26.62	0.015	−0.19
	33.33	33.85	0.072	1.55

Limits of detection (LOD) and limits of quantification (LOQ) were defined as three and ten times the ratio between the standard deviation of y-axis intercept and the slope of the calibration curve, respectively. RME: relative mean error.