Figure 6.

DG–KO cells showed decreased emerin levels, aberrant nuclear morphology and increased nucleus-centrosome distance. (A) Emerin levels were assessed in WT and DG–KO C2C12 cells by western blotting, using antibodies against β-DG, emerin and actin (loading control). Results correspond to mean +SEM of three separate experiments, with a p-value showing significant differences (unpaired t-test); (B) WT and DG–KO C2C12 cells were immunolabeled for emerin and counterstained with DAPI to decorate nuclei. The percentage of cells with aberrant nuclear morphology was calculated from three independent experiments (n = 150 nuclei), with a p-value showing significant differences (unpaired t-test). Scale bar, 10 µm; (C) WT and DG–KO C2C12 cells were double stained with anti-γ-tubulin antibodies and DAPI to decorate centrosomes and nuclei, respectively. Typical nuclei showing centrosome positioning are shown; scale bar, 10 µm. Nucleus-centrosome distance were measured in overlaid images using Leica Application Suite, Advanced Fluorescence Lite imaging processing software. Data in the graph correspond to the mean ± SD of triplicate experiments (n = 300 nuclei), with p-value denoting significant difference (student’s t-test).