Figure 5.

IQ induced apoptosis via mitochondrial ROS production in A549 cells. (A) The A549 cells were treated with IQ for 24 h. The cells were stained with annexin V-PI and analyzed via FACS analysis. (B) The bar graph is shown in panel A. (C) The expression of caspase 3, caspase 9, and PARP in the cells was analyzed via Western blotting. GAPDH was used as a loading control. (D–F) The cells were pretreated with MitoTEMPO for 1 h and then, treated with IQ for 18 h. (D) The cells were analyzed via MTT assay. (E) The cells were examined via annexin V-PI analysis. (F) The bar graph is shown in panel E. The experiments of panel B, D, and F were performed at least three times, and data are shown as the mean ± SD. Statistical analyses were performed using Student’s t-test. **, p < 0.05 and ***, p < 0.001 compared with the control.