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. 2020 Aug 25;21(17):6110. doi: 10.3390/ijms21176110

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Lysosome associated membrane protein 2 (LAMP-2) localization in osteoclast and osteoblasts. LAMP-2 (visualized with alexa-488 (green), localization in (A) Confocal image of a wild type osteoclast (OC) on a cortical bone slice. The green LAMP-2 label is mainly present in the area surrounded by actin (visualized with phalloidin-alexa-633, in blue). This area is considered as the ruffled border area. Nuclei were stained with propidium iodide (red). (B) Wild-type (left) and LAMP-2-/y (right) osteoblasts (OB) cultured on plastic. Images made by a converted fluorescence microscope. The green LAMP-2 label is present throughout the cytoplasm of wild type cells, but completely absent in the LAMP-2-/y cells. Nuclei were stained with di-amidino-2-phenylindole-dihydrochloride (DAPI).