Skip to main content
. 2020 Aug 25;21(17):6110. doi: 10.3390/ijms21176110

Figure 3.

Figure 3

Osteoclast formation and activity in macrophage colony stimulating factor (M-CSF) and the receptor activator of nuclear kappa β ligand (RANKL) stimulated bone marrow cultures. (A) Number of isolated bone marrow cells from WT and LAMP-2-/y. Calvariae of LAMP-2-/y mice contained more marrow cells than the WT calvariae (n = 6 ± SD, *** p < 0.001). The number of marrow cells isolated from LAMP-2-/y long bones is slightly higher than in WT long bones. (n = 6 ± SD, * p < 0.05). Number of multinucleated cells, with more than 2 nuclei, formed after 6 days of culture with M-CSF and RANKL. Marrow cells were obtained from calvariae and long bones of wild type and LAMP-2 -/y mice. (B) The number of multinucleated cells generated on bone is shown. (C) The number of multinucleated cells generated on plastic (n = 6 ± SEM, * p < 0.05). (D). Scanning electron micrographs of the resorptive activity of bone marrow cells from WT and LAMP-2-/y mice cultured on cortical bone slices. In both cultures resorption pits were formed (blue arrows).