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. 2020 Aug 19;21(17):5962. doi: 10.3390/ijms21175962

Figure 1.

Figure 1

Suppression of Kctd17 in zebrafish impairs ciliogenesis in Kupffer’s vesicle and induces situs inversus (A) Staining of acetylated alpha-tubulin in the cilia of Kupffer’s vesicle at 12 h post-fertilization (hpf) of wild-type (control) or Kctd17 knockout (KO) zebrafish. (B) Distribution of the cilia length in Kupffer’s vesicle at 12 hpf in control or Kctd17 KO or knockdown (KD) zebrafish. (C) Representative in vivo images of control and Kctd17 KO zebrafish at 3 days post-fertilization (dpf). Control and Kctd17 KO zebrafish show rightward and leftward looping of the heart, respectively. Please see Video S1. (D) At 3 dpf, rightward and leftward looping of the heart is observed in about 95% of the control zebrafish and about 20% of the Kctd17 KO zebrafish, respectively. Scale bar: 20 μm (A), 200 μm (C).