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. 2020 Aug 21;21(17):6038. doi: 10.3390/ijms21176038

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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The ESI-MSⁿ mass spectra of [ECA]_n-core OS identified in E. coli R2 LOS preparation: (a) in fraction 3 (negative-ion mode), inset the Bio-Gel P-10 elution profile; (b) in fraction 4 (negative-ion mode); (c) in fraction 5 (negative-ion mode); (d) in fraction 5 (positive-ion mode); (e) Positive-ion mode ESI-MS² spectrum of the ion at m/z 1278.88 (+2) attributed to [ECA]-core-P₃-Etn glycoform, where core stands for Glc₃-GlcNAc-Gal-Hep₃-Kdo oligosaccharide.. The interpretation of ions is shown in Table 3. The most informative ions are colored in red. The mark # stands for non-interpreted ions.