Table 3.
Oligosaccharide Structure | Calculated Monoisotopic Mass (Da) | Observed Ion (m/z) | Calculated Ion (m/z) | Interpretation of the Ion |
---|---|---|---|---|
Fraction 3 (negative mode) | ||||
[ECA]5 | 3054.12 | nd/1526.06 | 1017.03/1526.05 | [M-3H]3−/[M-2H]2− |
[ECA]5-OAc | 3096.13 | nd/1547.06 | 1031.04/1547.06 | [M-3H]3−/[M-2H]2− |
[ECA]3-Glc3-GlcNAc-Gal-Hep3-Kdo-P3-Etn | 3770.16 | 1255.69/nd | 1255.71/1884.07 | [M-3H]3−/[M-2H]2− |
[ECA]3-Glc3-GlcNAc-Gal-Hep3-Kdo-P4-Etn | 3850.12 | 1282.34/nd | 1282.37/1924.06 | [M-3H]3−/[M-2H]2− |
[ECA]3-Glc3-GlcNAc-Gal-Hep3-Kdo-P4-Etn2 | 3893.17 | 1290.66/nd | 1290.71/1936.57 | [M-H2O-3H]3−/[M-H2O-2H]2− |
[ECA]3-Glc3-GlcNAc-Gal-Hep3-Kdo-P4-Etn2 | 3893.17 | 1296.69/nd | 1296.72/1945.58 | [M-3H]3−/[M-2H]2− |
[ECA]3-Glc3-GlcNAc-Gal-Hep3-Kdo-P4-Etn2 | 3893.17 | 1304.03/nd | 1304.04/1956.56 | [M-3H, Na]3−/[M-2H, Na]2− |
[ECA]3-Glc3-GlcNAc-Gal-Hep3-Kdo-P5-Etn2 | 3973.13 | 1323.38/nd | 1323.37/1985.56 | [M-3H]3−/[M-2H]2− |
[ECA]4-Glc3-GlcNAc-Gal-Hep3-Kdo-P2 | 4254.37 | 1417.06/nd | 1417.12/2126.18 | [M-3H]3−/[M-2H]2− |
[ECA]4-Glc3-GlcNAc-Gal-Hep3-Kdo-P3-Etn | 4377.38 | 1458.08/nd | 1458.12/2187.68 | [M-3H]3−/[M-2H]2− |
[ECA]4-Glc3-GlcNAc-Gal-Hep3-Kdo-P4-Etn2 | 4500.39 | 1499.07/nd | 1499.12/2249.19 | [M-3H]3−/[M-2H]2− |
Fraction 4 (negative mode) | ||||
[ECA]4 | 2446.90 | nd/1222.44 | 814.63/1222.44 | [M-3H]3−/[M-2H]2− |
[ECA]2-Glc3-GlcNAc-Gal-Hep3-Kdo-P3-Etn | 3162.94 | 1053.30/1580.47 | 1053.30/1580.46 | [M-3H]3−/[M-2H]2− |
[ECA]2-Glc3-GlcNAc-Gal-Hep3-Kdo-P4-Etn | 3242.90 | 1079.66/1620.44 | 1079.96/1620.44 | [M-3H]3−/[M-2H]2− |
[ECA]2-Glc3-GlcNAc-Gal-Hep3-Kdo-P4-Etn2 | 3285.94 | 1094.31/1641.94 | 1094.31/1641.96 | [M-3H]3−/[M-2H]2− |
[ECA]2-Glc3-GlcNAc-Gal-Hep3-Kdo-P4-Etn2 | 3285.94 | 1101.94/1652.91 | 1101.63/1652.95 | [M-3H, Na]3−/[M-2H, Na]2− |
[ECA]2-Glc3-GlcNAc-Gal-Hep3-Kdo-P5-Etn2 | 3365.91 | 1120.94/1681.90 | 1120.96/1681.95 | [M-3H]3−/[M-2H]2− |
[ECA]3-Glc3-GlcNAc-Gal-Hep3-Kdo-P3 | 3727.12 | 1241.39/nd | 1241.36/1862.55 | [M-3H]3−/[M-2H]2− |
[ECA]3-Glc3-GlcNAc-Gal-Hep3-Kdo-P3-Etn | 3770.16 | 1255.03/nd | 1255.71/1884.07 | [M-3H]3−/[M-2H]2− |
[ECA]3-Glc3-GlcNAc-Gal-Hep3-Kdo-P4-Etn | 3850.12 | 1282.38/nd | 1282.37/1924.06 | [M-3H]3−/[M-2H]2− |
[ECA]3-Glc3-GlcNAc-Gal-Hep3-Kdo-P4-Etn2 | 3893.17 | 1296.67/nd | 1296.72/1945.58 | [M-3H]3−/[M-2H]2− |
Fraction 5 (negative/positive modes) | ||||
Glc3-GlcNAc-Gal-Hep3-Kdo-P3 | 1905.45 | nd/951.73 | 634.14/951.72 | [M-3H]3−/[M-2H]2− |
Glc3-GlcNAc-Gal-Hep3-Kdo-P3-Etn | 1948.49 | nd/973.27 | 648.49/973.24 | [M-3H]3−/[M-2H]2− |
Glc3-GlcNAc-Gal-Hep3-Kdo-P4-Etn | 2028.46 | nd/1004.22 | 669.14/1004.21 | [M-H2O-3H]3−/[M-H2O-2H]2− |
Glc3-GlcNAc-Gal-Hep3-Kdo-P4-Etn | 2028.46 | nd/1013.23 | 675.15/1013.22 | [M-3H]3−/[M-2H]2− |
Glc3-GlcNAc-Gal-Hep3-Kdo-P4-Etn2 | 2071.50 | nd/1025.73 | 683.49/1025.73 | [M-H2O-3H]3−/[M-H2O-2H]2− |
Glc3-GlcNAc-Gal-Hep3-Kdo-P4-Etn2 | 2071.50 | nd/1034.74 | 689.49/1034.74 | [M-3H]3−/[M-2H]2− |
[ECA]-Glc3-GlcNAc-Gal-Hep3-Kdo-P3-Etn | 2555.71 | 850.88/1276.83 | 850.90/1276.85 | [M-3H]3−/[M-2H]2− |
[ECA]-Glc3-GlcNAc-Gal-Hep3-Kdo-P4-Etn | 2635.68 | 871.57/1307.82 | 871.55/1307.82 | [M-H2O-3H]3−/[M-H2O-2H]2− |
[ECA]-Glc3-GlcNAc-Gal-Hep3-Kdo-P4-Etn | 2635.68 | 877.63/1316.83 | 877.55/1316.83 | [M-3H]3−/[M-2H]2− |
[ECA]-Glc3-GlcNAc-Gal-Hep3-Kdo-P4-Etn2 | 2678.72 | 885.92/1329.31 | 885.89/1329.35 | [M-H2O-3H]3-/[M-H2O-2H]2− |
[ECA]-Glc3-GlcNAc-Gal-Hep3-Kdo-P4-Etn2 | 2678.72 | 891.88/1338.31 | 891.90/1338.35 | [M-3H]3−/[M-2H]2− |
[ECA]-Glc3-GlcNAc-Gal-Hep3-Kdo-P4-Etn2 | 2678.72 | 899.25/1349.31 | 899.22/1349.34 | [M−3H, Na]3−/[M−2H, Na]2− |
[ECA]-Glc3-GlcNAc-Gal-Hep3-Kdo-P5-Etn2 | 2758.69 | 912.56/1369.29 | 912.55/1369.33 | [M-H2O-3H]3−/[M-H2O-2H]2− |
[ECA]-Glc3-GlcNAc-Gal-Hep3-Kdo-P5-Etn2 | 2758.69 | 918.53/1378.31 | 918.56/1378.34 | [M-3H]3−/[M-2H]2− |
[ECA]-Glc3-GlcNAc-Gal-Hep3-Kdo-P5-Etn2 | 2758.69 | 925.89/1389.31 | 925.88/1389.32 | [M-3H, Na]3−/[M-2H, Na]2− |
Glc3-GlcNAc-Gal-Hep3-Kdo-P3-Etn | 1948.49 | nd/975.20 | 650.50/975.25 | [M+3H]3+/[M+2H]2+ |
Glc3-GlcNAc-Gal-Hep3-Kdo-P3-Etn | 1948.49 | nd/986.18 | 657.83/986.24 | [M+3H, Na]3+/[M+2H, Na]2+ |
Glc3-GlcNAc-Gal-Hep3-Kdo-P4-Etn2 | 2071.50 | nd/1027.65 | 685.50/1027.75 | [M-H2O+3H]3+/[M-H2O+2H]2+ |
Glc3-GlcNAc-Gal-Hep3-Kdo-P4-Etn2 | 2071.50 | nd/1036.74 | 691.51/1036.76 | [M+3H]3+/[M+2H]2+ |
[ECA]-Glc3-GlcNAc-Gal-Hep3-Kdo-P3-Etn | 2555.71 | 852.88/1278.88 | 852.91/1278.86 | [M+3H]3+/[M+2H]2+ |
[ECA]-Glc3-GlcNAc-Gal-Hep3-Kdo-P3-Etn | 2555.71 | 860.21/1289.84 | 860.24/1289.85 | [M+3H, Na]3+/[M+2H, Na]2+ |
[ECA]-Glc3-GlcNAc-Gal-Hep3-Kdo-P3-Etn | 2555.71 | nd/1300.75 | 867.57/1300.85 | [M+3H, 2Na]3+/[M+2H, 2Na]2+ |
[ECA]-Glc3-GlcNAc-Gal-Hep3-Kdo-P4-Etn | 2635.68 | 879.50/1318.75 | 879.57/1318.85 | [M+3H]3+/[M+2H]2+ |
[ECA]-Glc3-GlcNAc-Gal-Hep3-Kdo-P4-Etn2 | 2678.72 | 887.75/1331.28 | 887.91/1331.36 | [M-H2O+3H]3+/[M-H2O+2H]2+ |
[ECA]-Glc3-GlcNAc-Gal-Hep3-Kdo-P4-Etn2 | 2678.72 | 893.75/1340.34 | 893.91/1340.37 | [M+3H]3+/[M+2H]2+ |
[ECA]-Glc3-GlcNAc-Gal-Hep3-Kdo-P4-Etn2 | 2678.72 | 901.13/1351.34 | 901.24/1351.35 | [M+3H, Na]3+/[M+2H, Na]2+ |
[ECA]-Glc3-GlcNAc-Gal-Hep3-Kdo-P5-Etn2 | 2758.69 | 920.76/1380.33 | 920.57/1380.35 | [M+3H]3+/[M+2H]2+ |
OAc—O-acetyl group.