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. 2020 Sep 3;21(17):6428. doi: 10.3390/ijms21176428

Figure 5.

Figure 5

Construction of mutants by using the CRISPR/Cas9 system. (A) Genomic location of guide sequences (sgRNA-a, sgRNA-c, and sgRNA-ac) targeting BcMF30a and/or BcMF30c. (B) The gene editing of BcMF30a and BcMF30c in pCA-sgRNA-ac transgenic T0 lines. CRISPR/Cas9: complementation and clustered regularly interspaced short palindromic repeat/CRISPR-associated 9, sgRNA: single-guide RNA, sgRNA-a: sgRNA targeting BcMF30a, sgRNA-c: sgRNA targeting BcMF30c, sgRNA-ac: sgRNA targeting BcMF30a and BcMF30c.