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. 2020 Sep 3;21(17):6428. doi: 10.3390/ijms21176428

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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The in vitro and in vivo germination tests of bcmf30a bcmf30c mature pollen. (A) Pollen germination frequencies of bcmf30a bcmf30c pollen in vitro. Only viable pollen was counted for pollen germination rate statistics. The values are the mean ± SD. (B) Control and bcmf30a bcmf30c mature pollen germinated in vitro for 4 h. (C) Aniline blue staining of pollen tubes in pistils after self-pollination and cross-pollination for 12 h. The arrows indicated the pollen tubes and the asterisk indicated the end position of pollen tubes. Bars = 100 μm.