Table 3.
Primers used in the experiments.
Primers Used in qPCR | |||
---|---|---|---|
Gene | Species Affiliation | Primer | Primer Sequence |
Transgene (HSVtk/GFP) | - | HSVtk-f90 | 5′-ATGGTCCAGACCCACGTCA-3′ |
GFP-R59 | 5′-GTGCCGGTGATGCGGCACT-3′ | ||
IGFBP2 | Human | IGFBP2-forE4 | 5′-AGATGTCTCTGAACGGGCAG-3′ |
IGFBP2-revE4.1 | 5′-AAGAGATGACACTCGGGGTC-3′ | ||
murine | mIGFBP2-For | 5′-AGATGTCTCTGAACGGACAG-3′ | |
mIGFBP2-Rev | 5′-GAGATGGCACTCGGGGTC-3′ | ||
PCNA | Human | PCNA-for | 5′-GCTCCATCCTCAAGAAGGTGT-3′ |
PCNA-rev | 5′-GCAAATTCACCAGAAGGCATC-3′ | ||
murine | mPCNA-for | 5′-GCTCCATCCTGAAGAAGGTG-3′ | |
mPCNA-rev | 5′-CAAATTCACCCGACGGCATC-3′ | ||
18S RNA | Human | 18S-for | 5′-CGCGGTTCTATTTTGTTGGT-3′ |
18S-rev | 5′-ATGCCAGAGTCTCGTTCGTT-3′ | ||
murine | m18S-for | 5′-TGCAATTATTCCCCATGAACG-3′ | |
m18S-rev | 5′-GCCTCACTAAACCATCCAATC-3′ | ||
GPI | Human | GPI-forE2 | 5′-GACCGCTTCAACCACTTCAG-3′ |
GPI-revE3 | 5′-CTCCGTCACCAGGTTCTTG-3′ | ||
EEF1A1 | Human | EEF1A1-forE1 | 5′-GACACGTAGATTCGGGCAAG-3′ |
EEF1A1-revE2 | 5′-GATACCACGTTCACGCTCAG-3′ | ||
Psmb7 | murine | Psmb7-forE1 | 5′-GCGGCTGTGTCGGTGTTTC-3′ |
Psmb7-revE3 | 5′-CCTTCAGTTGCTCTCGTGTC-3′ | ||
Rab1b | murine | Rab1b-forE4 | 5′-CTGGTCAGGAGCGGTTCAG-3′ |
Rab1b-revE5 | 5′-TCTTGGTGGTGAGGTCACTC-3′ | ||
Primers Used for Obtaining Lentiviral Constructs | |||
Primer | Primer Sequence | ||
LV_For_L | 5′-CCGAGCGCTGTCGACGATATCACGCGTGG-3′ | ||
LV_Rev_L | 5′-GTCCCACGCGTGATATCGTCGACAGCGC-3′ | ||
Primers Used to Determine Viral Integration | |||
comLV1 for | 5′-CCACCAAGGCAAAGAGAAGA-3′ | ||
comLV1 rev | 5′-CTCCCAAGAACCCAAGGAAC-3′ | ||
Arf1-ForE3 | 5′-AGCTTCACCGTGTGGGATG-3′ | ||
Arf1-RevE5 | 5′-AGTTCCTGTGGCGTAGAGAG-3′ | ||
Additional Primers | |||
T7 | 5′-TAATACGACTCACTATAGGG-3′ | ||
Sp6 | 5′-CGATTTAGGTGACACTATAG-3′ | ||
IGFBP2-Mlu-R | 5′-ATTACGCGTCTGGCGGTCGGCAGCGC-3′ | ||
IGFBP2-Sal-F | 5′-TAAGTCGACTAGACGGGTCTGAAACTC-3′ | ||
IGFBP2-F634 | 5′-GGCAGGTTTTGCGGGGCAC-3′ | ||
IGFBP2-R135+ | 5′-CAGCCCACTCTCGGCAGCAT-3′ |