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. 2020 Aug 24;21(17):6098. doi: 10.3390/ijms21176098

Table 3.

Primers used in the experiments.

Primers Used in qPCR
Gene Species Affiliation Primer Primer Sequence
Transgene (HSVtk/GFP) - HSVtk-f90 5′-ATGGTCCAGACCCACGTCA-3′
GFP-R59 5′-GTGCCGGTGATGCGGCACT-3′
IGFBP2 Human IGFBP2-forE4 5′-AGATGTCTCTGAACGGGCAG-3′
IGFBP2-revE4.1 5′-AAGAGATGACACTCGGGGTC-3′
murine mIGFBP2-For 5′-AGATGTCTCTGAACGGACAG-3′
mIGFBP2-Rev 5′-GAGATGGCACTCGGGGTC-3′
PCNA Human PCNA-for 5′-GCTCCATCCTCAAGAAGGTGT-3′
PCNA-rev 5′-GCAAATTCACCAGAAGGCATC-3′
murine mPCNA-for 5′-GCTCCATCCTGAAGAAGGTG-3′
mPCNA-rev 5′-CAAATTCACCCGACGGCATC-3′
18S RNA Human 18S-for 5′-CGCGGTTCTATTTTGTTGGT-3′
18S-rev 5′-ATGCCAGAGTCTCGTTCGTT-3′
murine m18S-for 5′-TGCAATTATTCCCCATGAACG-3′
m18S-rev 5′-GCCTCACTAAACCATCCAATC-3′
GPI Human GPI-forE2 5′-GACCGCTTCAACCACTTCAG-3′
GPI-revE3 5′-CTCCGTCACCAGGTTCTTG-3′
EEF1A1 Human EEF1A1-forE1 5′-GACACGTAGATTCGGGCAAG-3′
EEF1A1-revE2 5′-GATACCACGTTCACGCTCAG-3′
Psmb7 murine Psmb7-forE1 5′-GCGGCTGTGTCGGTGTTTC-3′
Psmb7-revE3 5′-CCTTCAGTTGCTCTCGTGTC-3′
Rab1b murine Rab1b-forE4 5′-CTGGTCAGGAGCGGTTCAG-3′
Rab1b-revE5 5′-TCTTGGTGGTGAGGTCACTC-3′
Primers Used for Obtaining Lentiviral Constructs
Primer Primer Sequence
LV_For_L 5′-CCGAGCGCTGTCGACGATATCACGCGTGG-3′
LV_Rev_L 5′-GTCCCACGCGTGATATCGTCGACAGCGC-3′
Primers Used to Determine Viral Integration
comLV1 for 5′-CCACCAAGGCAAAGAGAAGA-3′
comLV1 rev 5′-CTCCCAAGAACCCAAGGAAC-3′
Arf1-ForE3 5′-AGCTTCACCGTGTGGGATG-3′
Arf1-RevE5 5′-AGTTCCTGTGGCGTAGAGAG-3′
Additional Primers
T7 5′-TAATACGACTCACTATAGGG-3′
Sp6 5′-CGATTTAGGTGACACTATAG-3′
IGFBP2-Mlu-R 5′-ATTACGCGTCTGGCGGTCGGCAGCGC-3′
IGFBP2-Sal-F 5′-TAAGTCGACTAGACGGGTCTGAAACTC-3′
IGFBP2-F634 5′-GGCAGGTTTTGCGGGGCAC-3′
IGFBP2-R135+ 5′-CAGCCCACTCTCGGCAGCAT-3′