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. 2020 Aug 25;21(17):6122. doi: 10.3390/ijms21176122

Figure 2.

Figure 2

Chrysophanol is not cytotoxic to Jurkat T cells under culture condition using RPMI medium. (A) Cell viability of Jurkat T cells treated with the indicated concentrations of chrysophanol in the indicated media at the indicated density for 24 h was assessed by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide). (B) The growth rate of Jurkat T cells treated with/without 40 μM chrysophanol in the indicated media at the indicated density for the indicated time was measured by cell counting. (C) Apoptotic population of Jurkat T cells treated with 40 μM chrysophanol in the indicated media at the indicated density for 24 h was acquired by flow cytometry. The mean value of three experiments ± SEM (standard error of mean) is presented. * p < 0.05 versus control (A,B) or between two indicated groups (C). CHR—chrysophanol, RPMI—Rosewell Park Memorial Institute, DMEM—Dulbecco’s Modified Eagle Medium.