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. 2020 Aug 28;21(17):6234. doi: 10.3390/ijms21176234

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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The effect of compound combinations on cell cycle progression in the MDA-MB-231 cell line. Cells were treated with 5-FU, 14B, or CX-4945, used separately or in combination for 48 h or 72 h, and then fixed and stained with PI. (A) Distribution of the cells in different phases of the cell cycle was determined by flow cytometry and analyzed with the MacCycle software to determine the percentage of cells in each phase of the cell cycle; (B) exemplary DNA histograms of the MDA-MB-231 cell line shown using the WinMDI 2.8 software.