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. 2020 Sep 3;21(17):6410. doi: 10.3390/ijms21176410

Figure 3.

Figure 3

The effect of GANT61 and LiCl treatment on the expression of GLI proteins. (A) GLI3 and (B) GLI1 protein expression after treatment with HH-GLI pathway inhibitors GANT61 and LiCl (G5—5 μM GANT61, G10—10 μM GANT61, LI10—10 mM LiCl, LI20—20 mM LiCl). (C) Coomassie gel stain of immunoprecipitation with GLI3 antibody (AF-3690, R&D) for two HNSCC cell lines treated with GANT61. In both cell lines, the IP successfully detects the GLI3FL and GLI3R bands. The unidentified GLI3 band is also faintly detected in both tested cell lines.