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The role of GSK3β phosphorylation in GLI3 protein activity. Western blot showing phosphorylation of GSK3β (pGSK) compared to total GSK levels in non-treated (NT) or GANT61 (10 μM) or LiCL (20 mM) treatment. All HNSCC cell lines show an increase in GSK3β phosphorylation with LiCl, and three of the lines also with GANT61 treatment. This effect is closely followed by the downregulation of GLI3FL.
