HH-GLI |
Hedgehog-Gli |
LiCl |
Lithium chloride |
HNSCC |
Head and neck squamous cell carcinoma |
HPV16 |
Human papilloma virus 16 |
CSC |
Cancer stem cells |
EGFR |
Epidermal growth factor receptor |
PI3K |
Phosphoinositol 3 kinase |
VEGF |
Vascular endothelial growth factor |
PTCH1 |
Patched 1 |
HH |
Hedgehog |
SMO |
Smoothened |
βTrCP1 |
Beta-transducin repeat containing protein 1 |
GSK3β |
Glycogen synthase 3 beta |
PKA |
Protein kinase A |
SUFU |
Suppressor of fused |
CK1 |
Casein kinase 1 |
GLI |
Glioma-associated oncogene homolog |
EMT |
Epithelial-mesenchymal transition |
NQC |
Nanoquinacrine |
DMEM:HAM’S |
Dulbecco’s Modified Eagle Medium: Ham’s F12 |
EMEM |
Eagle’s Minimum Essential Medium |
FBS |
Fetal bovine Serum |
MTT |
3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide |
PBS |
Phosphate Buffered Saline |
RIPA |
Radioimmunoprecipitation assay buffer |
BCA |
Bicinchonic Acid |
PAA |
Polyacrylamide |
TBST |
Tris-Buffered Saline, 0.1% Tween® 20 Detergent |
HRP |
Horseradish Peroxidase |
TENN |
50 mM Tris, 5 mM EDTA, 150 mM NaCl, 0.5% NP-40, pH 8.0 |