Figure 10.

Pro10-1D is a target of toll-like receptor 4 (TLR4) and downregulates MAPK and NF-κB translocation in LPS-stimulated RAW264.7 cells. (A) Immunoblot analysis revealed the suppression of LPS-mediated TLR4, total/phospho-JNK, total/phospho-ERK, and cytosolic and nuclear translocation of NF-κB in RAW264.7 cells treated with peptides (20 μM) and LPS (50 ng/mL). β-actin was used as an internal control. (B) Graphical data represent the densitometry analysis of respective proteins, quantified by ImageJ. The values are expressed as the mean ± SEM of three independent experiments and are statistically significant, based on one-way ANOVA (Tukey’s post hoc test, ** p < 0.01, and *** p < 0.001; ns, not significant).