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. 2020 Aug 23;25(17):3832. doi: 10.3390/molecules25173832

Table 4.

The elution gradient for the simultaneous analysis of the plant extract combination.

No Time (min) ddH2O (Acetic Acid) Acetonitrile
1 0 84.5% (0.3%) 14.5%
2 8 74.5% (0.3%) 24.5%
3 16 50.0% (0.04%) 50.0%
4 24 40.0% (0.04%) 60.0%