Table 1.
Quantitative trait loci (QTLs) identified in chromosome 5 in the different assays with the recombinant inbred line (RIL) population derived from the cross between TGR-1551 and the cultivar ‘Bola de Oro’, phenotyped for resistance to Cucurbit yellow stunting disorder virus and genotyped by sequencing.
| Trait 1 | Interval 2 | Nearest Marker 3 | Kruskal–Wallis | Composite Interval Mapping | ||
|---|---|---|---|---|---|---|
| Mean BO 4 | Mean TGR 5 | LOD 6 | R 2,7 | |||
| S2009 (Scale 0–9) |
46.9–59.3 cM 6,810,744–23,928,244 bp |
S5_16214321 | 4.93 | 2.65 | 6.4 | 0.27 |
| S2010 (Scale 0–9) |
54.8–59.3 cM 16,214,321–23,928,244 bp |
S5_22526564 | 6.70 | 2.09 | 18.5 | 0.62 |
| S2011 (Scale 0–9) |
52.5–58.1 cM 14,759,610–22,875,514 bp |
S5_16214375 | 6.16 | 0.71 | 17.9 | 0.47 |
| S2012 (Scale 0–5) |
52.5–58.1 cM 14,759,610–22,875,514 bp |
S5_16214375 | 4.26 | 1.89 | 12.3 | 0.34 |
| S2013 (% infection) |
52.7–59.9 cM 14,759,610–24,296,585 bp |
S5_20703035 | 43.26 | 19.27 | 10.5 | 0.30 |
| qRT-PCR2012 | 68.8–79.3 cM 25,036,350–27,121,114 bp |
S5_26193386 | 28.26 | 35.17 | 16.1 | 0.49 |
| qRT-PCR2013-5 | 68.0–74.3 cM 25,036,35–26,193,386 bp |
S5_26193386 | 26.88 | 35.06 | 19.9 | 0.53 |
| qRT-PCR2013-8 | 65.5–74.3 cM 24,791,006–26,193,386 bp |
S5_25229866 | 26.09 | 33.08 | 15.1 | 0.51 |
| Response | 66-78 cM 24,957,179–26,993,475 bp |
S5_25229866 | 0.94 | 0.33 | 12.7 | 0.44 |
1 Trait: symptom evaluation for the five assays (S2009, S2010, S2011, S2012, and S2013; scale for evaluation is indicated for each assay), qRT-PCR virus titer in 2012 (qRT-PCR2012) and 2013 (the evaluations took place 5 weeks post inoculation for qRT-PCR2013-5 and 8 weeks post inoculation for qRT-PCR2013-8), and response (classification of each RIL as resistant or susceptible considering evaluation data gathered in all the assays). See Section 3 for details. 2 Interval position of the putative QTL on the genetic and physical maps according to an LOD drop of 2. The physical position (v3.6.1) is defined by the position of the markers flanking the QTL interval. 3 Closest marker to the LOD peak. Significance level in the Kruskal–Wallis test was 0.0001 for all the markers. 4 Mean of the genetic class TGR-1551 for the corresponding marker. 5 Mean of the genetic class ‘Bola de Oro’ for the corresponding marker. 6 Higher logarithm of the odds score. 7 Percentage of phenotypic variance explained by the QTL.