Figure 3.

Tube formation of HUVECs using Matrigel basement membrane matrix. Different concentrations of PG or UPG were evaluated in the presence of 10 ng/mL VEGFA for 6 h at 37 °C in CO₂ atmosphere_. Graphical representation of tube formation for prodigiosin (A) and undecylprodigiosin (B). The angiogenesis score (scale without unit) was calculated considering the number of sprouting cells, connected cells and polygons, and the complexity of the formed mesh. Data are expressed as mean ± SD. ^a, p < 0.05; ANOVA with Dunnett’s post hoc test. (C) Representative photographs after 6-h treatment with 32 nM prodigiosin and 32 nM undecylprodigiosin. The results were confirmed in three independent experiments (3 replicates per experiment).