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. 2020 Sep 1;21(17):6354. doi: 10.3390/ijms21176354

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Panning of the phage-displayed synthetic Fab libraries on hYKL-40. (a) Monitoring of the phage titers over three rounds (R2–R4) of panning. Black and gray bars indicate the ratio of the phage output to the input titers, presented as a percentage (%), from panning on immobilized hYKL-4 (black, hYKL-40(+)) and non-immobilized hYKL-40 (gray, hYKL-40(−)) surfaces. The ratio of the output to the input (%) = (phage output titer ÷ phage input titer) × 100. (b) Phage ELISA performed on the immobilized hYKL-40 surfaces of seven Fab phage clones (left) and their selection frequency (%) over the panning (right). The selection frequency of a unique clone (%) = (Number of unique clones ÷ Total number of phage ELISA positives) × 100. Fab: Antigen-binding fragment; ELISA: enzyme-linked immunosorbent assay.