Figure 3.

Production and characterization of anti-hYKL-40 IgGs. (a) SDS-PAGE analysis of three human anti-hYKL-40 IgGs, H1 (IgG), H2 (IgG), and H4 (IgG), purified from the culture media of Expi293F cells, which were transiently transfected with heavy- and light-chain expression vectors. β-MER (+) and β-MER (−) indicate with and without the reducing reagent (β-mercaptoethanol), respectively. (b) Soluble ELISA of serially diluted H1 (IgG), H2 (IgG), and H4 (IgG) on immobilized hYKL-40 surfaces to measure their apparent affinities (EC₅₀, nM). (c) Size-exclusion chromatography analysis of H1 (IgG), H2 (IgG), and H4 (IgG). The positions of the molecular mass markers, shown as kDa, on the retention time x-axis are shown above the peaks.