Table 1.
Physicochemical properties of human anti-hYKL-40 monoclonal antibodies (mAbs).
| Clones | Yield (mg/L Culture) | Tm (°C) | Monomericity (Mon/Agg.) | EC50 (nM) | KD (M) |
|---|---|---|---|---|---|
| H1 (Fab) | 1.8 | 76.5 | Mon. | 2.3 | n.d. |
| H2 (Fab) | 0.2 | 75.5 | Mon. | 4.0 | n.d. |
| H3 (Fab) | 0.2 | n.d. | n.d | n.f. | n.d. |
| H4 (Fab) | 0.5 | n.d. | n.d | n.f. | n.d. |
| H7 (Fab) | 0.6 | n.d. | n.d | n.f. | n.d. |
| H1 (IgG) | 0.8 | 73.7 | Mon. | 0.5 | 5.0 × 10−11 |
| H2 (IgG) | 5.9 | n.d. | Mon. | 0.3 | n.d. |
| H4 (IgG) | 21.5 | n.d. | Mon. | 13.6 | n.d. |
| H5 (IgG) | 1.0 | n.d. | Mon. | 327.4 | n.d. |
| H6 (IgG) | 1.7 | n.d. | Mon. | 69.4. | n.d. |
| H7 (IgG) | 17.4 | n.d. | Mon. | 371.3 | n.d. |
Fab: Antigen-binding fragment; IgG: immunoglobulin G; n.d.: not determined; Tm: melting temperature; EC50: half maximal effective concentration; KD: equilibrium dissociation constant; Mon.: monomer; Agg.: Aggregate; n.f.: not fittable.