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. 2020 Aug 26;21(17):6172. doi: 10.3390/ijms21176172

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Comparison of tri-lineage differentiation potential of DPSCs and UC-MSCs by real-time RT-PCR. (a) Quantitative analysis of mRNA expression for osteogenesis related genes (osteocalcin, osteopontin, Runx2) in DPSCs (left) and UC-MSCs (right). (b) Quantitative analysis of mRNA expression for chondrogenesis related genes (Acan, Col10A1, Col2A1, Sox9) in DPSCs (left) and UC-MSCs (right). (c) Quantitative analysis of mRNA expression for adipogenesis related genes (CEBPα, PPARγ) in DPSCs (left) and UC-MSCs (right). Cells were cultured in a StemPro osteogenesis differentiation kit, StemPro chondrogenesis differentiation kit, and StemPro adipogenesis differentiation kit for 7, 14, and 21 days, respectively. Fold differences in expression (2^−ddCt) of analysed genes in control cells cultured in standard cell culture medium (undifferentiated) were calculated as 1.0 and marked by a solid line. Graphs present different scales. Results are presented as mean ± standard error of the mean (SEM), n = 3 (every sample prepared for each DPSCs line derived from each donor were run in duplicates); t-test, (*) p < 0.05 vs. undifferentiated cells.