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. 2020 Aug 26;21(17):6172. doi: 10.3390/ijms21176172

Figure 5.

Figure 5

Comparison of cardiomyogenic and endothelial differentiation potential of DPSCs and UC-MSCs by Real-Time PCR. (a) Quantitative analysis of mRNA expression of cardiomyogenesis related genes (Gata-4, Nkx2.5, Myl2c) in DPSCs (left) and UC-MSCs (right). Cells were cultured in DMEM/F12 supplemented with 2% FBS and 10 ng/mL basic fibroblast growth factor (bFGF), 10 ng/mL vascular endothelial growth factor (VEGF) and 10 ng/mL transforming growth factor β1 (TGF-β1) for 7, 14 and 21 days. (b) Quantitative analysis of mRNA expression for endothelial related genes (Gata-2, Tie-2, VE-cadherin) in DPSCs (left) and UC-MSCs (right). Cells were cultured in EGM-2MV endothelial cell growth medium for 7, 14 and 21 days. Fold differences in the expression (2−ddCt) of analysed genes in control cells cultured in standard cell culture medium (undifferentiated) were calculated as 1.0 and marked by a solid line. Graphs present different scales. Results are presented as mean ± SEM, n = 3 (every sample prepared for each DPSCs line from each donor was run in duplicate); t-test, p < 0.05 vs. undifferentiated cells.