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The effect of exposure to various concentrations of bile mixture (BM) and different pH on the viability of esophageal epithelial cell lines. Het-1A (A) and EPC2 (B) cells were treated for 30 min with BM at concentrations ranging from 0 to 800 μM. After 24 h, MTT assay was used to determine cell viability. Significant changes (p < 0.05) in cell viability after BM treatment at pH 5.0 as compared to the treatment with BM in regular medium were indicated by asterisk (*).
