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. 2020 Sep 3;21(17):6436. doi: 10.3390/ijms21176436

Figure 3.

Figure 3

The effect of treatment with bile mixture (BM), acidified medium alone or acidic BM for one, three and six consecutive days on KRT4, 15, 8 and 18 mRNA expression in EPC2 cell line. EPC2 cell line underwent 30 min of daily incubation with BM at concentration of 100 µM, acidified medium to pH 5.0 alone or BM (100 µM) in acidified medium (pH 5.0) for one, three or six consecutive days and expression of KRT4, KRT15, KRT 8 and KRT18 mRNA was analyzed by real-time PCR (A–D). PCR reaction was performed in duplicates and quantified using ACTB/GAPDH as reference genes. Data from three independent experiments are shown as the mean ± SEM. An asterisk (*) indicates a significant change as compared with untreated control cells (p < 0.05). Significant change (p < 0.05) in gene expression as compared with one day exposure for respective treatment regime is indicated by hash (#). A cross (+) indicates a significant change as compared with cells after three days of treatment (p < 0.05).