Table 5.
Summary of alterations in expression of squamous and columnar epithelium-specific genes observed in human biopsies and in vitro using Het-1A and EPC2 cells and in vivo EGDA rat model. A vertical up arrow (↑) indicates upregulation of mRNA expression in Barrett’s metaplasia as compared with samples without Barrett’s metaplasia/untreated control cells/intact rats (background in orange); a vertical down arrow (↓) indicates downregulation of mRNA expression in Barrett’s metaplasia as compared with samples without Barrett’s metaplasia/untreated control cells/intact rats (background in blue); a horizontal left right arrow (↔) indicates no changes in mRNA expression (background in dark grey); n.a. indicates no amplification; n.d. not determined (background in light grey).
| Gene Symbol | Type of Epithelium | In Vitro Models | In Vivo Model | Human Biopsies | |
|---|---|---|---|---|---|
| Het-1A | EPC2 | ||||
| KRT1 | squamous | n.a. | ↓ | ↑ | ↓ |
| KRT4 | squamous | n.a. | ↓ | ↓ | ↓ |
| KRT5 | squamous | n.a. | ↓ | ↑ | ↓ |
| KRT6 | squamous | ↔ | ↓ | n.d. | ↓ |
| KRT13 | squamous | n.a. | ↓ | ↓ | ↓ |
| KRT14 | squamous | n.a. | ↓ | ↑ | ↓ |
| KRT15 | squamous | ↓ | ↓ | ↓ | ↓ |
| KRT16 | squamous | ↔ | ↓ | n.a. | ↓ |
| KRT23 | squamous | n.a. | ↓ | ↔ | ↓ |
| KRT24 | squamous | n.a | ↓ | n.a. | ↓ |
| KRT7 | columnar | ↔ | ↑ | ↑ | ↑ |
| KRT8 | columnar | ↑ | ↔ | ↑ | ↑ |
| KRT18 | columnar | ↔ | ↑ | ↑ | ↑ |
| KRT20 | columnar | n.a. | n.a. | ↑ | ↑ |
| TFF1 | columnar | n.a. | n.a. | ↓ | ↑ |
| TFF2 | columnar | n.a. | n.a. | n.a. | ↑ |
| TFF3 | columnar | ↑ | ↑ | ↑ | ↑ |
| VIL1 | columnar | ↑ | n.a. | ↔ | ↑ |
| MUC2 | columnar | ↑ | n.a. | ↑ | ↑ |
| MUC3 | columnar | n.a. | n.a. | n.a. | ↑ |
| MUC5B | columnar | n.a. | n.a. | n.a. | |
| MUC6 | columnar | n.a. | n.a. | n.a. | ↑ |
| MUC13 | columnar | ↑ | ↔ | ↑ | ↑ |