Table 1.

Thickness and mechanical properties of native and decellularized ovine aortic valve leaflets and walls measured from stress-strain curves obtained via uniaxial tensile testing.

Treatment Method	Tissue	Thickness, mm	Direction	Ultimate Tensile Strength, MPa	Strain at Fracture, %	Young’s Modulus, MPa
None	Leaflet	0.23 ± 0.04	Circ.*	4.19 ± 1.44	56.3 ± 14.4	12.01 ± 1.08
	Wall	1.5 ± 0.35	Circ.	0.43 ± 0.20	29.4 ± 5.0	2.83 ± 1.16
			Long.**	0.35 ± 0.11	36.6 ± 15.1	2.71 ± 1.37
scCO2 (15 MPa)-Ethanol	Leaflet	0.13 ± 0.01	Circ.	5.99 ± 1.54	22.5 ± 11.7	109.13 ± 15.24
	Wall	1.15 ± 0.17	Circ.	1.13 ± 0.20	55.0 ± 20.0	4.02 ± 2.02
			Long.	0.23 ± 0.04	18.5 ± 6.2	3.63 ± 0.93
scCO2 (25 MPa)-Ethanol	Leaflet	0.19 ± 0.04	Circ.	12.76 ± 6.09	31.5 ± 15.0	104.43 ± 29.57
	Wall	1.66 ± 0.21	Circ.	1.01 ± 0.58	54.2 ± 14.4	3.85 ± 0.68
			Long.	0.31 ± 0.10	26.7 ± 12.2	3.99 ± 0.76
Alkali-salt + scCO2 (10 MPa)	Leaflet	0.10 ± 0.01	Circ.	24.50 ± 9.19	46.6 ± 25.5	2043.60 ± 903.98
	Wall	1.30 ± 0.25	Circ.	0.46 ± 0.08	55.0 ± 25.0	1.04 ± 0.46
			Long.	0.14 ± 0.04	32.5 ± 7.5	0.90 ± 0.18
Alkali-salt + scCO2 (15 MPa)	Leaflet	0.10 ± 0.01	Circ.	47.38 ± 18.36	53.3 ± 24.4	4500.00 ± 2039.12
	Wall	1.42 ± 0.33	Circ.	0.60 ± 0.13	36.3 ± 3.8	1.62 ± 0.49
			Long.	0.19 ± 0.01	37.5 ± 5.0	0.69 ± 0.04
Detergent	Leaflet	0.15 ± 0.03	Circ.	5.52 ± 2.28	68.4 ± 32.3	4.79 ± 2.25
	Wall	1.26 ± 0.11	Circ.	0.48 ± 0.19	76.0 ± 21.7	0.31 ± 0.04
			Long.	1.27 ± 0.08	41.7 ± 17.2	2.46 ± 0.59
Detergents + scCO2 (15 MPa)	Leaflet	0.12 ± 0.03	Circ.	76.27 ± 27.24	15.0 ± 5.0	207.98 ± 38.54
	Wall	1.41 ± 0.15	Circ.	0.39 ± 0.06	22.5 ± 8.3	1.40 ± 0.67
			Long.	0.15 ± 0.04	42.5 ± 10.0	0.44 ± 0.06
Detergents + scCO2 (25 MPa)	Leaflet	0.12 ± 0.01	Circ.	20.43 ± 8.54	25.0 ± 5.0	106.20 ± 56.11
	Wall	1.16 ± 0.25	Circ.	0.87 ± 0.03	40.0 ± 10.0	1.15 ± 0.55
			Long.	0.15 ± 0.04	56.7 ± 32.2	0.51 ± 0.27

* Circumferential; ** Longitudinal.