Figure 1.

Pro-migratory effects of four porcine-derived collagen matrices on primary human oral cell types. Migration of human periodontal ligament (hPDL) (a,b) and human oral fibroblasts (hOF) (a,c) cells toward non-crosslinked collagen matrix (NCM), crosslinked collagen matrix (CCM), dried acellular dermal matrix (DADM), and hydrated acellular dermal matrix (HADM) matrices was evaluated by transwell migration assay using filters with 8 μm pore size. (a) Representative images of fixed and stained cells that have migrated to the lower side of the filter in each of the experimental groups. Scale bar, 500 μm. (b,c) Bar charts presenting quantification of cell migration in the absence (Ctrl) or presence of collagen matrices by measuring the area on the lower side of the filter covered with migrated cells. Data represent means ± SD from three independent experiments performed with three different cell donors, in duplicates. Significant differences to the respective control unless otherwise indicated, *** p < 0.001, * p < 0.05, ns: not significant.