Figure 4.

Increased expression of proliferative marker genes in two oral cell types cultured on four different collagen scaffolds. hPDL (a) and hOF (b) cells were grown on four different collagen matrices (NCM, CCM, DADM, and HADM) for 1 and 3 days before total RNA was isolated and analyzed for the expression of proliferative marker genes (MYBL2, BUB1, PLK1, MKI67, PCNA, CCNE1, CCND1, and CCNB1) by qRT-PCR. Controls (Ctrl) represent cells of each cell type plated on cell culture plastic in the absence of a collagen matrix. Values normalized to GAPDH are expressed relative to the values of control cells at day 1 (1d). Data represent means ± SD from three independent experiments performed with three different cell donors for each of the two cell types. Significant differences to the respective controls at day 1 unless otherwise indicated, *** p < 0.001, ** p < 0.01, * p < 0.05.