Figure 5.

Increased expression of adhesive marker genes in two oral cell types grown on four different collagen matrices. hPDL (a) and hOF (b) cells were cultured on four different collagen matrices (NCM, CCM, DADM, and HADM) for 9 h followed by an extensive wash for complete removal of nonadherent cells before total RNA was extracted and analyzed for the expression of adhesive marker genes (FN1, VCL, CD44, and ICAM1) by qRT-PCR. Controls (Ctrl) represent cells of each cell type grown on cell culture plastic in the absence of a collagen matrix. Values normalized to GAPDH are expressed relative to the values of control cells. Data represent means ± SD from three independent experiments performed with three different cell donors for each of the two cell types. Significant differences to the respective controls, *** p < 0.001, ** p < 0.01, * p < 0.05.