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. 2020 Aug 26;21(17):6170. doi: 10.3390/ijms21176170

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Identification of mutations generated by the clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9 (CRISPR/Cas9) system. (A) The mutation efficiency of sgRNAs (single guided RNAs), (B) The mutation rate in T₀ (Transgenic) generation, (C) sequencing chromatograms of WT (wild type) and homozygous mutant lines for Target 1 and Target 2, and (D) the alignment of sequences for T₁, T₂, and T₃ generations, respectively. The target sequence is painted in yellow, while the PAM (protospacer adjacent motif) is in the green background, and insertions/deletions are represented by red hyphens and letters. The analysis was carried out in three replications for each line.

Identification of mutations generated by the clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9 (CRISPR/Cas9) system. (A) The mutation efficiency of sgRNAs (single guided RNAs), (B) The mutation rate in T₀ (Transgenic) generation, (C) sequencing chromatograms of WT (wild type) and homozygous mutant lines for Target 1 and Target 2, and (D) the alignment of sequences for T₁, T₂, and T₃ generations, respectively. The target sequence is painted in yellow, while the PAM (protospacer adjacent motif) is in the green background, and insertions/deletions are represented by red hyphens and letters. The analysis was carried out in three replications for each line.