Table 1.
Primary antibodies used in the study.
| Description | Concentration | Product No. and Manufacturer | |
|---|---|---|---|
| PARP1 | Mouse monoclonal | 1:500 | #556494, BD Bioscences, Franklin Lakes, NJ, USA |
| p53 (DO-1) | Mouse monoclonal | 1:500 | sc-126, Santa Cruz, Dallas, TX, USA |
| p-p53 (S15) | Rabbit polyclonal | 1:500 | #9284, Cell Signaling Technology, Danvers, MA, USA |
| p21 WAF1/CIP1 | Mouse monoclonal | 1:500 | P1484, Sigma-Aldrich, St. Louis, MO, USA |
| ATM (Y170) | Rabbit monoclonal | 1:500 | ab32420, Abcam, Cambridge, UK |
| p-ATM (S1981) | Mouse monoclonal | 1:500 | ab36810, Abcam, Cambridge, UK |
| ATR | Rabbit polyclonal | 1:500 | #2790, Cell Signaling Technology, Danvers, MA, USA |
| p-ATR (S428) | Rabbit polyclonal | 1:500 | #2853, Cell Signaling Technology, Danvers, MA, USA |
| Ku70 (E-5) | Mouse monoclonal | 1:500 | sc-17789, Santa Cruz, Dallas, TX, USA |
| ὙH2AX (S139) | Mouse monoclonal | 1:1000 | ab26350, Abcam, Cambridge, UK |
| ὙH2AX (S139) | Rabbit monoclonal | 1:500 | #9718, Cell Signaling Technology, Danvers, MA, USA |
| 53BP1 | Rabbit polyclonal | 1:500 | NB100, Novus Biologicals, Centennial, CO, USA |
| Ki67 | Rabbit polyclonal | 1:500 | ab15580, Abcam, Cambridge, UK |
| SQSTM1/p62 | Mouse monoclonal | 1:1000 | #610832, BD Bioscences, Franklin Lakes, NJ, USA |
| SQSTM1/p62 | Guinea pig polyclonal | 1:500 | GP62-C, Progen, Heidelberg, Germany |
| LC3B | Rabbit polyclonal | 1:500 | L7543, Sigma-Aldrich, St. Louis, MO, USA |
| ULK1 (D8H5) | Rabbit monoclonal | 1:250 | #8054, Cell Signaling Technology, Danvers, MA, USA |
| p-ULK1 (S757) | Rabbit monoclonal | 1:250 | #14202, Cell Signaling Technology, Danvers, MA, USA |
| m-TOR (7C10) | Rabbit monoclonal | 1:500 | #2983, Cell Signaling Technology, Danvers, MA, USA |
| p-m-TOR (S2448) | Rabbit monoclonal | 1:500 | #5536, Cell Signaling Technology, Danvers, MA, USA |
| p70S6K | Rabbit monoclonal | 1:500 | #2708, Cell Signaling Technology, Danvers, MA, USA |
| p-p70S6K (T389) | Rabbit monoclonal | 1:500 | #9234, Cell Signaling Technology, Danvers, MA, USA |
| p-S6 (S235/236) | Rabbit monoclonal | 1:500 | #4858, Cell Signaling Technology, Danvers, MA, USA |
| 4EBP1 | Rabbit polyclonal | 1:500 | #9452, Cell Signaling Technology, Danvers, MA, USA |
| p-4EBP1 (T37/46) | Rabbit monoclonal | 1:500 | #2855, Cell Signaling Technology, Danvers, MA, USA |
| p-AMPK(T172) | Rabbit monoclonal | 1:500 | #2531, Cell Signaling Technology, Danvers, MA, USA |
| AMPK | Rabbit monoclonal | 1:250 | #2532, Cell Signaling Technology, Danvers, MA, USA |
| p-Akt(S473) | Rabbit monoclonal | 1:2000 | #4060, Cell Signaling Technology, Danvers, MA, USA |
| Akt | Rabbit monoclonal | 1:1000 | #4691, Cell Signaling Technology, Danvers, MA, USA |
| LAMP-2 | Mouse monoclonal | 1:500 | #14-1078-82, eBioscience, Thermo Fisher Scientific, Waltham, MA, USA |
| TFEB | Rabbit polyclonal | 1:250 | #4240, Cell Signaling Technology, Danvers, MA, USA |
| p-TFEB (S142) | Rabbitpolyclonal | 1:500 | ABE-1971, Merck Millipore, Burlington, MA, USA |
| Rubicon | Rabbit monoclonal | 1:500 | #8465, Cell Signaling Technology, Danvers, MA, USA |
| GAPDH | Mouse monoclonal | 1:50,000 | MAB374, Merck Millipore, Burlington, MA, USA |