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. 2020 Aug 21;21(17):6037. doi: 10.3390/ijms21176037

Figure 1.

Figure 1

Flow cytometry of epidermal growth factor receptor (EGFR)-transfected and EGFR-expressing cell lines using anti-EGFR antibodies EMab-17 and EMab-51. (A) CHO-K1 and CHO/EFGR cells were subjected to Western blotting analysis with an anti-EGFR or anti-β-tubulin antibody. (B) CHO-K1 and EGFR-transfected CHO-K1, CHO/EGFR cells were treated with 1 μg/mL of anti-EGFR antibodies, EMab-51 (Left; blue) and EMab-17 (Right; red). After treatment with the anti-EGFR antibodies, the cells were treated with Alexa Fluor 488-conjugated anti-mouse IgG; black line, negative control. Fluorescence data were collected using a Cell Analyzer EC800. (C) CRC cell lines with or without KRAS mutations were treated with EMab-51 (Top; blue) and EMab-17 (Bottom; red). Yellow highlights indicate the KRAS status of CRC cell lines. Data were analyzed as in (B).