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. 2020 Aug 26;21(17):6157. doi: 10.3390/ijms21176157

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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LP-A96 induced exosomes are properly loaded with RNAs. (A) Exosomes purified from subconfluent HCE-T cells after 36 h of incubation with either 1 μM LP-A96 in basal media or complete media shows stimulated exosome biogenesis and release by LP-A96 treatment. These exosomes were subjected to RNA isolation. (B,C) Elevated small RNA (0~270 nucleotides) and miRNA (10~40 nucleotides) amount reflects an increase in total exosome amount shown in (A). (D) Small RNAs, (E) miRNAs, and (F) miRNA-to-small RNA ratio per exosome particle under two different treatment conditions are similar. n = 3. Mean ± SD. * p < 0.05, ns: non-significant.