Figure 5.

LP-A96-mediated exosome biogenesis does not affect cell viability in corneal epithelial cells. (A) Brightfield images of HCE-T cells at 48 h post-treatment. The appearance of LP-A96 treated HCE-T cells at 48 h was typical of that observed throughout the 3-day incubation period, while all other treatments did not affect the appearance of the cells. Yellow arrows indicate possible perinuclear compartments. (B) Cell viability upon 1 μM LP-A96 treatment was quantified alongside other treatments. 50 μM Digitonin was used as a cytotoxic agent. All experiments were performed when cells were subconfluent (30%). At 24 h, LP-A96 vs. A96 treatment: p = 0.012*; LP-A96 vs. dPBS: p = 0.052. A one-way ANOVA followed by multiple comparisons was used for statistical comparison. * p < 0.05. Mean ± SD.