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. 2020 Aug 26;21(17):6157. doi: 10.3390/ijms21176157

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Contact lens-delivered LP-A96 maintains its activity in corneal epithelial cells. (A) Concentration-dependent adsorption of LP-A96 to contact lenses over a 24 hr period. (B) Time-dependent adsorption of 2 mg/mL LP-A96 to contact lenses. (C) Time-dependent release of LP-A96 from contact lenses. (D) Study design of the contact lens-mediated delivery of LP-A96 to a scratch (gap) generated corneal epithelial cell monolayer. (E) Gap closure activity of HCE-T cells incubated with contact lenses loaded with different agents. (F) After gap closure activity measurement, exosomes were purified from culture media and quantified using nanoparticle tracking analyzer. (F) Exosome abundance in the culture media after 24 hr period. A one-way ANOVA followed by multiple comparisons was used for statistical comparison. CM indicates complete media. * p < 0.05, ns: non-significant. Mean ± SD.