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. 2020 Aug 27;21(17):6195. doi: 10.3390/ijms21176195

Figure 4.

Figure 4

Expression of the miR-302/367 cluster is regulated upon knockdown of ADAR1. (A) Mapping of miR-302/367 cluster components on the UCSC genome browser (genome.ucsc.edu, GRCh37/hg19). (B) Comparison of sequence alignment of miR-302/367 cluster components; hsa-miR-302a-3p, hsa-miR-302b-3p, hsa-miR-302c-3p, and hsa-miR-302d share identical seed sequences (marked as red) that match the 3′-UTR of IRF9. (C) miR RT-PCR result showing reduced miR-302b-3p, miR-302c-3p, and miR-302d in ADAR1 KD cells compared with the control. (D) miR RT-PCR result showing reduced miR-302a-5p in ADAR1 KD cell line compared with the control. (E) Precursor miRNA RT-PCR result showing reduced precursor miR-302a level in ADAR1 KD cells compared with the control. ** p < 0.01, *** p < 0.001.