Figure 4.
Changes of apoptosis (caspase activity) of UVC and/or CHW09 treatments in oral cancer cells. Cells (Ca9-22 and CAL 27) were pretreated with UVC irradiation UVC (0 and 30 J/m2) and/or post-treated with CHW09 (control, 25, and 50 µg/mL) for 24 h. (A) Representative pattern of pancaspase analysis for UVC and/or CHW09 treatment. (+) in the right side of each panel indicates the pancaspase (+) (%). (B) Statistics in Figure 4A. Data, mean ± SD (n = 3 independent experiments). For multiple comparison, significance of the difference was examined by one-way ANOVA and the Tukey HSD Post Hoc Test. *, p < 0.0001. * without line indicates the significance between control and UVC30. * with line indicates the significance between UVC30/CHW09 and others. (C) Cas 3/7 activity in CHW09 and/or UVC-treated Ca9-22 and CAL 27 cells. Cas 3/7 activity was determined by ELISA assays. (D) Expressions of cleaved form to PARP (c-PARP) in CHW09 and/or UVC-treated Ca9-22 cells in the absence and presence of Z-VAD pretreatment (100 µM for 2 h).