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. Author manuscript; available in PMC: 2020 Sep 21.
Published in final edited form as: Nat Metab. 2020 Mar 16;2(3):278–289. doi: 10.1038/s42255-020-0184-y

Fig. 2 |. Metrnl deletion alters immune cell migration and phenotype in injured muscle.

Fig. 2 |

a, Total counts of CD45+ cells in muscle throughout 7 d of recovery (n = 5 per group). Asterisks indicate the means that are significantly different from controls: *P = 0.0029, **P = 0.0016, ***P = 0.006. b, Immune inflammatory profile showing CD45+, CD11b+, Gr1− cells stained with Ly6c to show pro- and anti-inflammatory status (n = 5 biologically independent samples). c, Quantification of percentage Ly6c+/− cells in uninjured and 1, 4 and 14 d post-injury, wild-type and Metrnl KO muscle (n = 4 or 5 per group). d, Metrnl protein expression in medium from cultured Ly6c+/− cells sorted 1 and 4 d after injury (n = 3 per group). e, Metrnl protein expression in the medium normalized to cell number (n = 3 per group). NS, not significant. Two-tailed, unpaired, Student’s t-test (a); one-way ANOVA with Tukey’s post hoc comparison (c); and multiple, two-tailed, unpaired, Student’s t-tests (d,e). Data are presented as mean ± s.e.m.