Figure A2.

The effect of hydrogen peroxide on cell viability of differentiated myotubes. (a) The cell viability of myotube with or without hydrogen peroxide (H₂O₂, 2 mM) was measured using Cell counting kit-8 (Dojindo, Rockville, MD, USA). Treatment of 2 mM H₂O₂ did not induce cell death of myotubes. (b) Representative images of Western blot analysis to determine protein expression levels of Cleaved PARP and Annexin V. Beta-actin was used as a loading control. Data are expressed as mean ± SEM.